First comprehensive proteomics analysis of lysine crotonylation in leaves of peanut ( <i>Arachis hypogaea</i> L.)

Lysine crotonylation is an important post-translational modification process. Most research in this area has been carried out on mammals and yeast, but there little it plants. In the current study, large-scale lysine crotonylome analysis was performed by a combination of affinity enrichment high-resolution LC-MS/MS analysis. Altogether, 6051 sites were identified 2508 protein groups. Bioinformatics showed that lysine-crotonylated proteins involved many biological processes, such as carbon fixation photosynthetic organisms, biosynthesis amino acids, ribosomes structure function. particular, subcellular localization 43% crotonylated located chloroplast. Twenty-nine associated with photosynthesis functional these reaction center, electron transport, ATP synthesis. Based results, further studies to expand suggested.